Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Fabrication of tissue‐engineered cell sheets by automated cell culture equipment

doi: 10.1002/term.2968

Figure Lengend Snippet: Results of fabricating oral mucosal epithelial cell sheets using the automated cell culture equipment

Article Snippet: For the automated culture test, we used human oral mucosal epithelial cells manufactured by CELLnTEC (Bern, Switzerland), Lot numbers ES1305033 and MC1507246.

Techniques: Cell Culture

Journal: Journal of Oncology

Article Title: Posttranslational Modifications of Rev-Erb α Protein and Abnormal Inflammatory Response in Gastric Cancer

doi: 10.1155/2022/6291656

Figure Lengend Snippet: Rev-erb α protein is specifically decreased human gastric cancer tissues. Human gastric cancer tissues (gastric cancer) and normal tissues adjacent to cancer (control) were used. (a) H&E staining was performed in gastric tissues with 400x magnification, and representative images are shown. (b) Immunofluorescent double-staining for Rev-erb α and a gastric mucosal epithelial cell marker Tff1 was performed with 200x magnification. Representative images shown are those that were captured by a fluorescence microscope. Arrows denote co-localization of Rev-erb α and Tff1. (c) Co-localization signals of Rev-erb α and Tff1 were analyzed. (d) IgG control staining was performed in gastric cancer tissues and adjacent normal tissues. Scale bar: 50 μ m. Mean ± SEM, N = 6. ∗∗ P < 0.01 vs the control group.

Article Snippet: Human gastric mucosal epithelial cells (GES-1) and human gastric cancer cell lines (BGC-823 and SGC-7901) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control, Staining, Double Staining, Marker, Fluorescence, Microscopy

Journal: Journal of Oncology

Article Title: Posttranslational Modifications of Rev-Erb α Protein and Abnormal Inflammatory Response in Gastric Cancer

doi: 10.1155/2022/6291656

Figure Lengend Snippet: Rev-erb α protein phosphorylation is unchanged in gastric cancer cells line BGC-823 and in MNU/ H. pylori -induced mouse gastric tumor tissues. (a, c) Levels of Rev-erb α protein phosphorylation were determined in normal human gastric mucosal epithelial cells (GES-1) and human gastric cancer cell lines (BGC-823) in the logarithmic growth phase by Western blot. (b, d) Rev-erb α protein phosphorylation at Ser55/59 and Thr275 was detected in normal control mice and MNU/ H. pylori -induced mouse gastric cancer tissues. Calnexin was used as a housekeeping control. Mean ± SEM, N = 4–6.

Article Snippet: Human gastric mucosal epithelial cells (GES-1) and human gastric cancer cell lines (BGC-823 and SGC-7901) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Journal of Oncology

Article Title: Posttranslational Modifications of Rev-Erb α Protein and Abnormal Inflammatory Response in Gastric Cancer

doi: 10.1155/2022/6291656

Figure Lengend Snippet: Rev-erb α protein SUMOylation and ubiquitination modifications are significantly increased in gastric cancer cells line BGC-823 and in MNU/ H. pylori -induced moues gastric tumor tissues. (a, b) Rev-erb α protein SUMOylation and ubiquitination modifications in normal human gastric mucosal epithelial cells (GES-1), human gastric cancer cell lines (BGC-823) (a), control mouse gastric tissues (Ctr), and MNU/ H. pylori -induced mice gastric cancer tissues (b) were detected by immunoprecipitation. (c, d) Ubiquitin, SUMO1, and SUMO2 were standardized with the corresponding Rev-erb α . Mean ± SEM, N = 4–5. ∗∗∗ P < 0.001 vs GES-1 or Ctr. IP: immunoprecipitation; IB: immunoblot.

Article Snippet: Human gastric mucosal epithelial cells (GES-1) and human gastric cancer cell lines (BGC-823 and SGC-7901) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Ubiquitin Proteomics, Control, Immunoprecipitation, Western Blot